Aug 18, 2018 - Phase contrast is used extensively in optical microscopy. In biology, it is employed in viewing unstained biological, making it possible to. We highly discourage buying the Chinese microscopes due to the problems that incur after the sale. Testing Results: This microscope was fully tested. Results are as follows: All functions were found to be in proper working order. Brightfield – Works fine and as expected. Phase Contrast – All objectives performed well under phase.
The optical path length is the product of the refractive index and the thickness between two points in an optical path. It is related to the transit time and the velocity of light. The differences in the optical path length lead to different velocities of the light waves when they pass through the specimen (i.e. phase shift). As a result differences in phase occur. A higher refractive index, compared to the surrounding medium, leads to a deceleration of the light wave and a retardation of its phase.
Interference describes the interaction of two waves with each other and the resulting formation of a new wave pattern following the principle of superposition. The relevant parameter for the interference of light waves is the light wave’s amplitude. If two waves interfere, the amplitude of the resulting light wave will be equal to the vector sum of the amplitudes of the two interfering waves.
If the amplitude of the resulting wave is increased, the interference will be described as constructive. This will be the case if either two wave crests or two wave troughs meet at the same point in time. It is also possible that a crest of one wave and a trough of another wave meet at the same point in time. This leads to a decreased amplitude of the resulting wave. The interference between these two waves is then called destructive.
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US $292.13 | Germany |